The heart of the company’s design is a custom-designed nanopore,
inserted into a polymer membrane that rests on top of a microwell. The
membrane has a high electrical resistance, and a voltage is applied so a
current passes through the nanopore. Each microwell has its own
electrode. A user would pour some purified DNA into the cartridge, where
it would flow over the membrane and through the nanopores. As the DNA
strand passes through a pore, each of its nucleotides interrupts the
current in a measurable way. This change in conductivity can be used to
identify the nucleotide.
Whole arrays of nanopores and their
microwells are embedded onto chips, using typical semiconductor
manufacturing techniques, and these are inserted into a disposable
cartridge. Each cartridge is built so the nanopores are tuned to sense
specific molecules — like DNA, or maybe proteins, drugs or other
compounds. A user inserts the cartridge into the sequencing node of
choice: either the GridION node, which looks like an old-school VCR, or
the MinION system, which is a slightly fat USB stick.
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